Abstract
Nowadays, the encapsulation of cytotoxic chemotherapeutic agents is attracting interest as a method for drug delivery. We hypothesized that the efficiency of helenalin might be maximized by encapsulation in β-cyclodextrin nanoparticles. Helenalin, with a hydrophobic structure obtained from flowers of Arnica chamissonis and Arnica Montana, has anti-cancer and anti-inflammatory activity but low water solubility and bioavailability. β-Cyclodextrin (β-CD) is a cyclic oligosaccharide comprising seven D-glucopyranoside units, linked through 1,4-glycosidic bonds. To test our hypothesis, we prepared β-cyclodextrin- helenalin complexes to determine their inhibitory effects on telomerase gene expression by real-time polymerase chain reaction (q-PCR) and cytotoxic effects by colorimetric cell viability (MTT) assay. MTT assay showed that not only β-cyclodextrin has no cytotoxic effect on its own but also it demonstrated that β-cyclodextrin- helenalin complexes inhibited the growth of the T47D breast cancer cell line in a time and dose-dependent manner. Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentration of β-cyclodextrin-helenalin complexes increased. β-Cyclodextrin-helenalin complexes exerted cytotoxic effects on T47D cells through down-regulation of telomerase expression and by enhancing Helenalin uptake by cells. Therefore, β-cyclodextrin could be superior carrier for this kind of hydrophobic agent.
Highlights
Breast cancer starts usually occurs in the inner lining of the lobules or milk ducts in breast
We investigate the inhibitory effect of β-Cyclodextrin- Helenalin complex on telomerase gene expression in T47D breast cancer cell line by q-PCR
Results for MTT assay T47D cells were exposed to variable concentrations of β-CD-Helenalin complex (1-1.8μM with six concentration) for 24, 48 and 72h and IC50 of 2,1.64 and 1.4 was obtained for 24, 48 and 72h, respectively .Our Data analysis of cytotoxicity assay showed that IC50 of β-CyclodextrinHelenalin complex on T47D breast cancer cell line was time and dose-dependent (Figure 4)
Summary
Breast cancer starts usually occurs in the inner lining of the lobules or milk ducts in breast. Inhibitory Effects of β-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR Results: MTT assay showed that β-cyclodextrin has no cytotoxic effect on its own and it demonstrated that β-cyclodextrinhelenalin complexes inhibited the growth of the T47D breast cancer cell line in a time and dose-dependent manner.
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