Abstract
The compound chalcon originally is extracted form some plant and herbs, the studies of the antiviral activity of this compound were done in two cell line cultures the L2OB and RD, the compound relatively non toxic to both cell lines of the concentration of 32?g/ml or less ,the compound have significantly anti poliovirus activity in both L2OB cell line and RD cell line, we find that the concentration of 0.03 ?g/ml or more inhibit the 100TCDID50 of the poliovirus .The therapeutic index(TI)used in this study to evaluate the drug activity ,( TI is the ratio of dose of drug which is just toxic to the cells to the does which is just inhibit the viral multiplication, if this index more than one the margin of safety of drug is according great ) .In this study the TI of chalcone against poliovirus is 266,therefore this compound if used in man have little or no side effect.
Highlights
Poliovirus infections of the nervous system have largely eliminated by immunization, the disease is caused by one of the poliovirus serotypes (1,2 and 3) which are belong to the family picornaviridae subdivision of enteroviruses group.This virus causes meningitis and infects the gray matter of the spinal cord,brain stem and cortex, poliovirus that causes a nonspecific gasteroenteritis, the nervous system invaded secondary especially the anterior horn cells in the lumber segments
Toxicity of chalcone was estimated by incorporating varying amount of the drug (1-12&ug/ml) in tissue culture maintenance media of RD cells and L20B cells and adaily microscopic examination of the cells for toxic effect such as floating cells, cells, cell granulation or any alteration of cell shape
Observations were recorded daily until the end of the incubation period (4 to 5 days), concentration of 32 μg/ml or greater were rapidly toxic while a concentration of 16 μg/ml were toxic on day 2.The concentration of 8 μg/ml or less showed no toxic effect to the cell culture used in this study(table 1)
Summary
Poliovirus infections of the nervous system have largely eliminated by immunization, the disease is caused by one of the poliovirus serotypes (1,2 and 3) which are belong to the family picornaviridae subdivision of enteroviruses group.This virus causes meningitis and infects the gray matter of the spinal cord,brain stem and cortex , poliovirus that causes a nonspecific gasteroenteritis, the nervous system invaded secondary especially the anterior horn cells in the lumber segments. We have presented in this study , one of the flavonoid compound (the Chalcone), such compound can be isolated from many herbs and plants such as beans , tomatoes , grape fruit and they have the ability to across the blood-Brain barrior[1,2,3,4]. The antiviral chalcone have an antiviral activity against rubella in tissue culture and against rhinovirus in vitro and in human [5, 6, 7]; it is nontoxic to cell, and has been shown in vitro using two cell lines L2OB and RD cells against poliovirus type 1 and 3 ; they are clearly and significantly showed an antiviral activity against poliovirus and by the using of therapeutic index [8, 9]. The need for antiviral compound that have the ability to across the blood-brain
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