Abstract
Tropical forest plants contain a variety of secondary metabolites, and plant aqueous extracts can be used as urease and nitrification inhibitors to improve nitrogen use efficiency and reduce the negative environmental effects. An incubation experiment was carried out to investigate the effects of aqueous extracts of 60 tropical forest plant species from 16 families on urea hydrolysis and nitrification. The results indicated that the aqueous extracts from 3 of the tropical forest plants (Aphanamixis polystachya, Toona sinensis, Vatica mangachapoi) belonging to meliaceae and dipterocarpaceae showed good inhibitory effects on urease, and the aqueous extracts from 5 of the tropical forest plants (Cryptocarya chinensis, Cryptocarya hainanensis, Toona sinensis, Khaya senegalensis, Hopea hainanensis) belonging to lauraceae, meliaceae and ipterocarpaceae showed good inhibitory effects on nitrification. The inhibitory effects of active substances in plants were affected by extract temperature, and the plants can be divided into three types by solubility and thermal stability. Toona sinensis had the most potential for development in this study as urea additive. The inhibitory effects of aqueous extracts of Toona sinensis leaves on urea hydrolysis and nitrification increased with increasing concentration of aqueous extracts, the strongest inhibitory effect on urease occurred after 2-3 d of incubation, and the strongest inhibitory effect on nitrification occurred after 2-3 weeks of incubation.
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