Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells.

Wei-An Mao,Khalid Rahman,Ying Ye,Li Wang,Jie Zhou,Jing-Yi Mao,Jian Zhang,Yuan-Yuan Sun

doi:10.1155/2016/6063475

Abstract

This study was designed to investigate the inhibitory effects of Angelica polysaccharide (AP) on activation of mast cells and its possible molecular mechanism. In our study, we determined the proinflammatory cytokines and allergic mediators in anti-DNP IgE stimulated RBL-2H3 cells and found that AP (50, 100, and 200 μg/mL) significantly decreased the release of histamine, β-hexosaminidase, leukotrienes C4 (LTC4), IL-1, IL-4, TNF-α, IL-6, and human monocyte chemotactic protein-1 (MCP-1/CCL2) (p < 0.05). In addition, Ca2+ entry was inhibited by treatment with AP. AP also downregulated the protein expressions of p-Fyn, p-Akt, p-P38, IL-4, TNF-α, and NF-κB p65 in both Fyn gene upregulated and normal RBL-2H3 cells (p < 0.05). Collectively, our results showed that AP could inhibit the activation of mast cells via suppressing the releases of proinflammatory cytokines allergic mediators, Gab2/PI3-K/Akt and Fyn/Syk pathways.

Highlights

Allergic disorders, such as eczema, allergic rhinitis, and asthma, are generally considered as intractable diseases threatening people’s health with an increasing prevalence in recent years [1, 2]
Co. (CA, USA); rats histamine, IL-1, TNF-α, IL-6, leukotrienes C4 (LTC4), β-hexosaminidase, and MCP-1/CCL2 ELISA kits were purchased from the Boster Co. (Wuhan, China); p-Fyn and Fyn primary antibodies were purchased from Abcam Co. (Cambridge, UK); p-Akt, Akt, pP38, P38, and NF-κB p65 primary antibodies were purchased from CST Co. (MA, USA); TNF-α, IL-4, and GAPDH primary antibodies were purchased from Santa Cruz Biotech. (CA, USA); BCA protein kit and horseradish peroxidase(HPR-) conjugated secondary antibodies were purchased from Beyotime Co. (Jiangsu, China); PVDF membrane was purchased from Millipore Biotech. (MA, USA)
Our results showed that Angelica polysaccharide (AP) at concentrations of 50, 100, and 200 μg/mL possessed significant histamine suppressing activities compared to the control group, in a concentration-dependent manner (p < 0.01) (Figure 1(b))

Summary

Introduction

Allergic disorders, such as eczema, allergic rhinitis, and asthma, are generally considered as intractable diseases threatening people’s health with an increasing prevalence in recent years [1, 2]. There is increasing evidence that mast cells play crucial roles in the development and pathogenesis of allergic diseases [3, 4]. When rechallenged by the same allergen, the mast cells would be activated and degranulated; subsequently, various proinflammatory cytokines and allergic mediators would be released, leading to systemic allergic reactions [6, 7]. Angelica sinensis (Oliv.) Diels, belonging to the family of Apiaceae, is one of the well-known and commonly used traditional Chinese medicines. In traditional Chinese medicine theory, A. sinensis is a widely and commonly used drug for treating blood deficiency, inflammatory and gynecological diseases, and so forth. Increasing investigations have demonstrated that the AP possesses a wide range of pharmacological activities on the immune and circulatory system of humans including antitumor activity, immunoregulatory effect, radioprotective effect, and inhibition of platelet aggregation [10, 11]

Methods

Results

Conclusion