Abstract
The aim of this study was to investigate the inhibitory effect of regulation of miR-122-MAP3K2 signal pathway on the hepatitis B cells. We detected the content of MAP3K2 from patients with HBV blood serum samples and analyzed the correlation between content of MAP3K2 and copies of HBV-DNA. Wound healing and Transwell assays were used to detect the function of cells from control group (wild type) and observer group (overexpresses miR-122). Secretion levels of HBsAg and MAP3K2 in the supernatant and level of MAP3K2 in cells were detected by ELISA and western blot, respectively. The results showed that there was a positive correlation between the copies of HBV-DNA and MAP3K2 in serum. In the assays involving detection of the number of HBV-DNA copies, the supernatant levels of HBsAg and MAP3K2, and the level of MAP3K2 in the cells, the rate of increase of these indicators significantly slowed as culture time. In conclusion, overexpression of miR-122 could inhibit the migration of hepatoblastoma cells; however, following transfection with miR-122, DNA synthesis and the secretion of HBsAg were inhibited. Overexpression of miR-122 can also downregulate MAP3K2. Consequently, we concluded that regulating the miR-122-MAP3K2 signaling pathway exerts an inhibitory effect in hepatitis B cells.
Highlights
Cirrhosis is an independent predictor of liver cancer development in patients with chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) infections (Bal and Onlen 2018, Cheng et al 2018)
It has been previously reported that there is a correlation between HBV infection and certain miRs, and this group of miRs should receive more attention due to their potential for acting as biomarkers for disease diagnosis (Rohan et al 2018, Burmi et al 2019, Ruiz Esparza-Garrido et al 2017). miR-122 is an miR abundant in the healthy liver, which plays a role in stimulating the translation of HBV and HCV RNA
A total of 27 patients with HBV enrolled in the study, arranged from May 2017 to May 2018, we analysed HBV-DNA copies and content of MAP3K2 in the blood serum samples and the result was showed in the Figure 1 and Table I, there was a positive correlation between the copies of HBVDNA and MAP3K2 in serum
Summary
Cirrhosis is an independent predictor of liver cancer development in patients with chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) infections (Bal and Onlen 2018, Cheng et al 2018). It has been previously reported that there is a correlation between HBV infection and certain miRs, and this group of miRs should receive more attention due to their potential for acting as biomarkers for disease diagnosis (Rohan et al 2018, Burmi et al 2019, Ruiz Esparza-Garrido et al 2017). A relationship between miR-122 and HBV infection has been previously reported, and, an increasing number of studies focus on its value as a candidate therapeutic biomarker for the assessment of liver cancer treatment (Bandopadhyay et al 2016, Wojcik et al 2016, Hoffmann et al 2012)
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