Abstract
Postprandial hyperglycemia plays an important role in the development of type 2 diabetes. Inhibition of alpha-amylase was led to a delay in breaks down of starch and glycogen and prevented a rapid rise in blood sugar. Alpha-amylase was isolated by gel filtration chromatography Sephadex G-75 from bovine pancreas. Then, total methanolic extracts of plants were prepared and IC50values of extracts on alpha-amylase were obtained and compared with acarbose IC50. The polyphenolic content of extracts and antioxidant capacity were determined by Folin-Ciocalteu test and DPPH test, respectively. The specific activity of alpha-amylase was 48.2 U/mg. For inhibition of alpha-amylase, IC50values ofH. persicum,Z. jujuba, and acarbose were 307, 827, and 113 μg/ml, respectively. For inhibition of DPPH radical, IC50values of extracts were 235 and 701 μg/ml. Total phenolic contents of methanol extracts were73.8±3.2and44.2±1.8 μg tannic acid equivalent/mg extract. Acarbose causes gastrointestinal symptoms and liver toxicity, butH. persicumandZ. jujubadecrease these side effects and prevent gastrointestinal disorders. Due to the high polyphenolic content and antioxidant capacity of these plants and significant inhibitory effect of the plants on alpha-amylase, these plants can be proposed for treatment of diabetic patients.
Highlights
Diabetes mellitus (DM) is a chronic metabolic disease characterized by hyperglycemia, resulting from insufficient or inefficient insulin secretion, with alterations in carbohydrate, lipid, and protein metabolism [1]
The results of this study showed that methanolic extracts of H. persicum and Z. jujuba have a high inhibitory effect on activity of alpha-amylase enzyme with IC50 307 and 867 μg/mL, respectively
The results showed that 0.5 mg/mL Luteolin inhibited 36% activity of alpha-glucosidase enzyme that this effect was stronger than acarbose
Summary
Diabetes mellitus (DM) is a chronic metabolic disease characterized by hyperglycemia, resulting from insufficient or inefficient insulin secretion, with alterations in carbohydrate, lipid, and protein metabolism [1]. Postprandial phase is characterized by a rapid increase in blood glucose and its high levels. These postprandial “hyperglycemic spikes” may be relevant to the pathophysiology of late diabetic complications that is recently receiving much attention [3]. Type 2 diabetes is caused by an imbalance between blood sugar absorption and insulin secretion. Postprandial hyperglycemia plays an important role in the development of type 2 diabetes [4]
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