Inhibitory effect of wheat bran feruloyl oligosaccharides on oxidative DNA damage in human lymphocytes

Abstract

The present work assessed the protective effect of feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides from wheat bran, against oxidative DNA damage in normal human peripheral blood lymphocytes induced by hydrogen peroxide (H2O2). The DNA damage was measured by using the single cell gel electrophoresis assay (comet assay). Lymphocytes were subjected to DNA damage by exposure to a range of H2O2 concentrations (10–200μmol/l). H2O2, at a concentration of 200μmol/l, resulted in nearly all cells being highly damaged. FOs showed no cytotoxicity and genotoxicity to normal human lymphocytes at the tested concentrations (10–500μmol/l). In addition, DNA damage in human lymphocytes induced by 100μmol/l H2O2 was inhibited by FOs in a concentration-dependent fashion with 91.1% inhibition of lymphocyte DNA damage at 500μmol/l as compared with the control. The results suggest that water-soluble FOs from wheat bran are able to enhance the ability of human lymphocytes to resist H2O2 induced oxidative damage.