Abstract
The effect of tetrandrine, a bis-benzylisoquinoline alkaloid, on dopamine biosynthesis in PC12 cells was investigated. Tetrandrine at a concentration of 3.0 microM decreased dopamine content by 59.4% (IC50 = 2.4 microM) and intracellular tyrosine hydroxylase (TH) activity was inhibited by the treatment of tetrandrine (49.8% inhibition at 3.0 microM) compared with control. We next examined the effects of tetrandrine on the kinetics of PC12 TH. The PC12 TH was obtained from PC12 cells with minor purification. Tetrandrine inhibited the PC12 TH activity by 40.6% at a concentration of 45 microM and exhibited noncompetitive inhibition on the enzyme using L-tyrosine as a substrate (Ki = 60.8 microM). These results suggest that the inhibition of TH activity by tetrandrine may partially contribute to the decrease in dopamine biosynthesis in PC12 cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
