Abstract
<p class="Abstract">The aim of this study was to examine the anticancer effects of schizophyllan (a -D-glucan) against the growth of rat CNS-1 glioma cells and preliminarily assess its effect on inducing apoptosis and blocking cell cycle. In order to evaluate its inhibitory effect, firstly MTT assay was conducted followed by annexin V/propidium iodide double staining or propidium iodide single staining, apoptosis and cell cycle using flow cytometry. All the experiments were carried in a dose- and time-dependent manner. Experimental results showed that treatment of 40 and 60 mg/L schizophyllan significantly increa-sed the apoptotic rate and blocked the cell cycle. In addition, increase in the proportion of cells in G0/G1 phase and decrease in the proportion of S-phase cells were also observed. Overall experimental studies suggest that schizo-phyllan can significantly inhibit the growth of rat CNS-1 glioma cells, in vitro and induced apoptosis and blocked the cell cycle.</p><p> </p>
Highlights
Malignant gliomas account for approximately 50% of all central nervous system tumors (Mahaley et al, 1989; Avgeropoulos et al, 1994)
3-(4,5-dimethylthiazol -2-yl)-2,5-diphenyltetrazolium bromide (MTT), annexin V/propidium iodide apoptosis detection kit and propidium iodide cell cycle assay kit were obtained from Santa Cruz Company (USA)
The results confirmed that schizophyllan can inhibit the growth of CNS-1rat glioma (Figure 2)
Summary
Malignant gliomas account for approximately 50% of all central nervous system tumors (Mahaley et al, 1989; Avgeropoulos et al, 1994). Mortality from malignant astrocytic gliomas, the most common intrinsic brain tumor in adult, still remains unacceptably high partly due to chemoresistance (Furnari et al, 2007). The highly lethal nature of this tumor partly originates from its invasive characteristics, which allow tumor cells to migrate and infiltrate eloquent areas making impossible the achievement of a radical surgery. Such invasive disease is, considered incurable using the treatment modalities presently available (Kislin et al, 2009). Traditional herbal therapy treatment has been regarded as one promising alternative to modern medicine (Tan et al, 2011), and various investigations of active herbal components with antitumor or anti-invasive potential and fewer adverse effects than chemotherapeutic agents have been conducted (Meiyanto et al, 2012)
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