Abstract
The calcium dependence of the inhibitory effect of prostaglandin E 2 on sympathetic neurotransmitter secretion was studied in isolated superfused field stimulated guinea-pig vas deferens, by determination of the nerve stimulation induced rise in efflux of total tritium after preincubation of the tissue with tritium-labelled 1-noradrenaline. Reuptake of noradrenaline was blocked by desipramine and normetanephrine, added to the medium. Reduction of the calcium in the superfusion medium strongly depressed the nerve stimulation induced rise in efflux of tritium. Addition of the alpha-adrenoceptor blocking drug phentolamine markedly raised the efflux of tritium on nerve stimulation, even at low calcium. The inhibitory effect of prostaglandin E 2 on nerve stimulation induced rise in efflux of tritium was inversely related to the calcium concentration. The results are consistent with the possibility that prostaglandin E 2 depresses neural secretion by facilitating efflux of intraaxonal calcium.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
