Inhibitory Effect of Osthole from Cnidium monnieri (L.) Cusson on Fusarium oxysporum, a Common Fungal Pathogen of Potato.

Hongli Zheng,Yahan Chen,Mingmin Zhao,Qiuli Guo,Hongyou Zhou,Hong Wei,Jianying Yue

doi:10.3390/molecules26133818

Abstract

Fusarium wilt of potato is one of the most common diseases of potato in China, and is becoming a serious threat in potato production. It has been reported that osthole from Cnidium monnieri (L.) Cusson can inhibit plant pathogens. Here, we test the anti-fungal activity of C. monnieri osthole against Fusarium oxysporum in potatoes. The results showed that at a concentration of 5 mg/mL, osthole was able to obviously inhibit mycelial growth of F. oxysporum. We found that osthole caused changes of mycelial morphology, notably hyphal swelling and darkening. Osthole significantly reduced the spore germination of Fusarium by 57.40%. In addition, osthole also inhibited the growth of other pathogens such as Fusarium moniliforme J. Sheld, Thanatephorus cucumeris Donk, and Alternaria alternata (Fr.) Keissl, but not Alternaria solani Jonesetgrout and Valsa mali Miyabe and G. Yamada. Our results suggest that osthole has considerable potential as an agent for the prevention and treatment of potato Fusarium wilt.

Highlights

Potato Fusarium wilt is a typical soil-borne disease that leads to severe wilt symptoms, resulting in plant death, which directly affects the yield and quality of potatoes [1,2,3]
The effect of osthole on F. oxysporum was examined on potato dextrose agar (PDA)
Compared with the control, the mycelial growth was significantly inhibited in plates containing osthole, as evidenced by mycelial growth was significantly inhibited in plates containing osthole, as evidenced by mycelial growth inhibition at 3 days after inoculation (Figure 1A)

Summary

Introduction

Potato Fusarium wilt is a typical soil-borne disease that leads to severe wilt symptoms, resulting in plant death, which directly affects the yield and quality of potatoes [1,2,3]. This disease is widely distributed worldwide and generally causes about. Fusarium wilt is mainly caused by either of five different Fusarium varieties, i.e., Fusarium oxysporum Schlecht, F. solani (Mart.) Sacc., F. moniliforme Sheld, F. sambucinum Fuckel, and. F. nivale (Fr.) Ces. F. oxysporum Schlecht, F. solani (Mart.) Sacc., and F. nivale (Fr.) Ces are the agents of potato Fusarium wilt in Inner Mongolia, China. The control of potato Fusarium wilt mainly relies on agricultural and chemical seed dressing methods

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Results

Conclusion