Abstract
Objective To observe the inhibition of immunoeffector cells on the.growth PC3 cells induced by dendritic cell fusion hybrids.Methods DCs were fused with PC3 cells and fusion cells (DC fusion hybrids) were marked with CD11c MicroBeads.In vitro,cytotoxin study,the immunoeffector cells were divided into A1 group (without DC stimulated),A2 group (DC stimulated),A3 group (DC fusion hybrids stimulated).During the in vivo study,18 nude mice were allocated to 3 groups:preventive group (Ⅱ),1-2 days before inoculating PC3,giving DC fusion hybrids activated immunoeffector cells; treated group (Ⅲ),giving DC fusion hybrids activated immunoeffector cells after emergence of implanted tumor in all nude mice; control group (Ⅰ),giving equivalent amount of 1640 cultured liquid.Results In vitro,the maximal killing rate was A3 group (76.6％).In vivo,12 mice developed implanted tumor on the day 12 in group Ⅰ and Ⅲ,separately; In group Ⅱ,on the 30th day,1 of the 6 mice developed implanted tumor.On the 45th day after development of implanted tumor,all the nude mice were.sacrificed and the tumor body weighed.There was significant difference among group Ⅰ,Ⅱ,Ⅲ (P ＜ 0.05).Conclusion DC fusion hybrids may play an important role in anti-tumor therapy. Key words: Dendritic cell; Pancreaticcarcinoma; Immunotherapy; Fusion hybrids
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