Abstract
目的探讨miRNA let-7a调控高迁移率族蛋白2（HMGA2）对人喉鳞癌细胞株TU212增殖的影响。 方法合成let-7a模拟体（let-7a mimics）并采用阳离子脂质体法瞬时转染入喉癌TU212细胞，裸鼠皮下注射TU212细胞，构建裸鼠移植瘤模型。RT-qPCR和Western blot法分别检测转染后TU212细胞及移植瘤内let-7a和HMGA2的表达。 结果在过表达let-7a的喉癌TU212细胞中，HMGA2的转录和翻译水平下调，细胞增殖能力降低。体外成瘤实验证实，与let-7a NC组和空白对照组相比，转染let-7a mimics的喉癌细胞TU212成瘤组织的质量和体积均显著下降；let-7a过表达的TU212成瘤组织中HMGA2的mRNA和蛋白水平均明显下调。 结论let-7a能显著抑制HMGA2的mRNA和蛋白表达，进而显著抑制喉癌细胞的增殖。
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