Inhibitory Effect of Methyl Jasmonate on Flowering and Elongation Growth in Pharbitis nil

Abstract

The effect of methyl jasmonate (JA-Me) on the floral bud formation and elongation growth in the short-day plant Pharbitis nil was investigated. The placing of 4-day-old seedlings of P. nil in a solution of JA-Me for a period of 24 h before an inductive (16 h or 14 h of darkness) night led to a dramatic reduction in the number of flower buds formed by the plant. Plants treated with JA-Me also totally lost their capacity to form a generative terminal bud. JA-Me applied after photoinduction does not inhibit flowering. Gibberellic acid (GA3) partly reverses the inhibitory effect of JA-Me. Plants treated simultaneously with JA-Me and GA3 formed about 3 flower buds more than plants treated with JA-Me only. JA-Me at a concentration of 10-7 M stimulates slightly, but at higher concentrations it inhibits root growth and shoot growth. A distinct lack of correlation between the effect of JA-Me on inhibition of flowering and shoot and root growth was noted. This indicates the independent action of JA-Me in controlling both processes.