Inhibitory effect of interfering the phosphorylation of epithelial growth factor receptor on breast cancer cell xenografts in nude mice

Abstract

Objective To investigate the effect of interfering phosphorylation of epithelial growth factor receptor (EGFR) on breast cancer cell xenografts in nude mice. Methods Breast cancer cell MDA-MB-231 xenograft model was established in nude mice and treated with adenovirus Ad5-hSulf1. The effect of hSulf-1 gene was examined on the inhibition of EGFR phosphorylation and xenograft growth. Results The recombinant adenovirus Ad5-hSulf1 expressed hSulf-1 factor and inhibited tumor growth. There was an obvious difference in tumor volume between Ad5-hSulf1 group (670.83±65.02) mm3 and blank control group (1 815.33±214.43) mm3 (P=0.000). The expression of hSulf-1 did not affect total EGFR expression but decreased p-EGFR expression level. Conclusion The expression of hSulf-1 can inhibit the EGFR phosphorylation and then inhibit the breast cancer xenograft growth. Key words: Breast cancer; Signal pathway; Epithelial growth factor receptor; Phosphorylation; Model, animal