Inhibitory effect of diosmetin on lipolysis and inflammation in adipocyte‐macrophage co‐culture model

Abstract

Obesity has become one of the most common metabolic syndromes and has come to pose a major worldwide threat to human health. Paracrine interaction between macrophages and adipocytes in obese tissue is thought to be a trigger of metabolic syndromes. Diosmetin is a flavone found in citrus fruits. Diosmetin has been shown to alleviate inflammatory cell infiltration and to repress the nuclear factor‐kappa B(NF‐κB) signaling pathway. However, not much is known about the anti‐inflammatory and anti‐lipolytic activities of diosmetin in co‐culture system of adipocytes and macrophages. The objective of this study was to evaluate the inhibitory effect of diosmetin on the inflammation and lipolysis in the interaction between adipocytes and macrophages. The secretion of inflammatory mediators such as nitric oxide, monocyte chemoattractant protein‐1, and tumor necrosis factor‐α increased in a co‐culture medium, however, diosmetin significantly decreased the inflammatory mediators. Diosmetin down‐regulated the expression of inducible nitric oxide synthase in co‐cultured macrophages and adipocytes. Diosmetin inhibited the phosphorylation of mitogen‐activated protein kinases and translation of NF‐κB. In addition, diosmetin suppressed the protein production of hormone sensitive lipase and fatty acid binding protein 4 and increased the mRNA expression of adiponectin in co‐cultured adipocytes. These results indicated that diosmetin inhibited inflammation and lipolysis in the crosstalk between adipocytes and macrophages and diosmetin‐containing foods could be used in dietary therapy for the prevention of obesity‐related metabolic diseases.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.