Abstract
Objective To investigate the feasibility of using cytokine-induced killer (CIK) cells as targeted vectors to deliver neo-interferon (mix) gene for colon cancer.Methods Replication-deficient adenovirus pDC359-mix + F35 carrying mix gene was generated.CIK cells were isolated and cultured.The infection efficiency of AdS-EGFP,Ad11-EGFP and Ad35-EGFP to CIK for 24 h and 48 h at MOI =100was determined by fluorescence microscope.CIK cells were infected by adenovirus pDC359-mix + F35 at MOI =0,1,10,100,1000 for 2,4,6,8 h,then the mix concentration in the culture supernatant of CIK cells was determined by using enzyme linked immunosorbent assay (ELISA).The inhibitory effect of mix and interferonα-2b at different concentrations of 0.001,0.01,0.1,1,10 μg/L on colon cancer cells HT29 and SW480 was detected by Sulforhodamine B (SRB).Cytotoxicity of CIK and CIK-mix in different ratio of efficiency to target 3∶1 and 10∶1 on HT29 and SW480 was tested by LDH release method in vitro.Results Replication-deficient adenovirus pDC359-mix + F35 was successfully generated as high infection efficiency as 80％ at MOI =100 for 48 h.At MOI =100,the mix concentration exceeded ( 8.96 ± 2.10) ng level at different periods.At 10 μg/L,the inhibitory rate of interferonα-2b and mix on HT29 and SW480 cells was 30.4％ and 28.6％,and 72.8％ and 70.1％ respectively.The cytotoxicity of CIK-mix on HT29 and SW480 was stronger by 20％ than CIK.Conclusion CIK cells with neo-interferon can exert synergistic antitumor effect. Key words: Neo-interferon mix; CIK cells; Adenovirus; Colon carcinoma
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