Inhibitory effect of chlorothiazide on glucose utilization by aorta.

Abstract

SummaryThe rate of utilization of glucose in vitro by normal dog aorta has been determined with various concentrations of chlorothiazide in the incubating Krebs'bicarbonate solution. Pieces of dog aorta totaling approximately 300 mg were incubated for 2 hr at 37° in 5.0 ml medium containing 200 mg glucose per 100 ml. Chlorothiazide added to the incubating solution caused a reduction in the rate of utilization of glucose by the aorta. As the chlorothiazide concentration in the incubating medium was increased there was a decrease in the glucose disappearance rate from the medium. At a chlorothiazide concentration of 1.0 mM, the mean rate of decrease of glucose from the medium was approximately one-half of the rate found when chlorothiazide was not present. This direct inhibitory effect of chlorothiazide on the utilization of glucose by dog aorta was evident even when the concentration of this drug was as low as 0.01 mM.