Abstract
Objective To observe the inhibitory effect of celecoxib on the growth of transplant tumor of medullary thyroid carcinoma in nude mice.Methods Forty mice were randomly divided into 4 groups (A,B,C and D):control group,celecoxib group,doxorubicin group and celecoxib ± doxorubicin group.All vaccination animal models were established by subcutaneous injection of 2 × 106 of human medullary thyroid carcinoma TT cells.The celecoxib was given 300 mg/(kg·d),and doxorubicin 2 mg/(kg·d).Tumor volumes and weight of mice were measured one week after administration when 0.5 cm tumor nodus appeared in the injection region,and tumor inhibition rate was calculated after 3 weeks.The expression levels of cyclooxegenase-2 (COX-2),matrix metalloproteinases-9 (MMP-9) and vascular endothelial growth factor (VEGF) in the vaccination tumor,liver and lung tissues were detected by using immunohistochemistry,Results The inhibition rate in groups A,B,C,and D was 0%,45.37%,49.71%,and 69.68% respectively,and the inhibitory effects in groups B,C and D were increased as compared with group A (P < 0.05),but there was no obvious difference among groups B,C and D.The expression levels of COX-2,MMP-9 and VEGF in group A were obviously higher.than in groups B,C and D (P < 0.05),but there was no significant difference among groups B,C and D (P > 0.05).In groups B,C and D,invasion of tumor cells in the liver and lung tissues was seen,and the expression of COX-2,MMP-9 and VEGF was positive.Conclusion Celecoxib can inhibit the growth of vaccination tumors of medullary thyroid carcinoma,and combination of celecoxib with doxorubicin can exert the synergistic effects. Key words: Celecoxib; Thyroid carcinoma; Cyclooxegenase-2 ; Matrix metalloproteinases-9 ; Vascular endothelial growth factor
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.