Abstract
Camptothecin (CPT) has anticancer, antiviral, and antifungal properties. However, there is a dearth of information about antibacterial activity of CPT. Therefore, in this study, we investigated the inhibitory effect of CPT on Acidovorax avenae subsp. avenae strain RS-2, the pathogen of rice bacterial brown stripe, by measuring cell growth, DNA damage, cell membrane integrity, the expression of secretion systems, and topoisomerase-related genes, as well as the secretion of effector protein Hcp. Results indicated that CPT solutions at 0.05, 0.25, and 0.50 mg/mL inhibited the growth of strain RS-2 in vitro, while the inhibitory efficiency increased with an increase in CPT concentration, pH, and incubation time. Furthermore, CPT treatment affected bacterial growth and replication by causing membrane damage, which was evidenced by transmission electron microscopic observation and live/dead cell staining. In addition, quantitative real-time PCR analysis indicated that CPT treatment caused differential expression of eight secretion system-related genes and one topoisomerase-related gene, while the up-regulated expression of hcp could be justified by the increased secretion of Hcp based on the ELISA test. Overall, this study indicated that CPT has the potential to control the bacterial brown stripe pathogen of rice.
Highlights
Camptothecin (CPT), a monoterpenoid indole alkaloid, exhibits promising anticancer and antimicrobial properties [1,2,3]
RS-2systemto CPT, CPT, we performed quantitative real-time primers forstrain secretion we performed quantitative real-time primers for secretion systemwe performed quantitative real-time primers for secretion systemor topoisomerase-related genes were validated in this study using the conventional PCR reaction or topoisomerase-related genes were in this study study using using the the conventional conventional PCR
Results from this study indicated that CPT solutions at 0.05, 0.25, and 0.50 mg/mL inhibited the growth of Aaa strain RS-2, and the inhibitory effect depends on the concentration, pH, and incubation time
Summary
Camptothecin (CPT), a monoterpenoid indole alkaloid, exhibits promising anticancer and antimicrobial properties [1,2,3]. (Aaa), the pathogen of rice bacterial brown stripe, Little is available about the avenae antibacterial activity of CPT against plant pathogenic which has received increased in China in(Aaa), recentthe years [9,10,11,12,13,14,15,16,17,18,19].ofMoreover, thisbrown pathogenic bacteria, in particular. Effective control method for the management of this pathogen is on the rise in rice-growing countries genome-wide in silico analysis has identified six topoisomerase-related genes and of theFortunately, world. Through measurement, electron observation, live/dead staining, Thegrowth aim of this study was transmission to examine the in vitromicroscopic antibacterial effect of CPT on Aaacell strain.
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