Abstract

Hydrogel antibacterial agent is an ideal antibacterial material because of its ability to diffuse antibacterial molecules into the decayed area by providing a suitable microenvironment and acting as a protective barrier on the decay interface. The biocompatibility and biodegradation make the removal process easy and it is already widely used in medical fields. However, there have been few reports on its application for controlling postharvest diseases in fruit. In this study, the Chitosan–silver (CS–Ag) complex hydrogels were prepared using the physical crosslinking method, which is used for controlling postharvest diseases in grape. The prepared hydrogels were stable for a long period at room temperature. The structure and surface morphology of CS–Ag composite hydrogels were characterized by UV-Vis, FTIR, SEM, and XRD. The inhibitory effects of CS–Ag hydrogel on disease in grape caused by P. expansum, A. niger, and B. cinerea were investigated both in vivo and in vitro. The remarkable antibacterial activity of CS–Ag hydrogels was mainly due to the combined antibacterial and antioxidant effects of CS and Ag. Preservation tests showed that the CS–Ag hydrogel had positive fresh-keeping effect. This revealed that CS–Ag hydrogels can play a critical role in controlling fungal disease in grapes.

Highlights

  • Published: 4 March 2022The decay process of fruit is caused by infection and reproduction of microorganisms [1]

  • The decayed grape was washed with sterile water, the filtrate was collected and diluted, and 100 μL diluted solution was uniformly spread on a petri dish containing potato dextrose agar (PDA) medium and cultured at 28 ◦ C for 7 d

  • Xerogel was freeze-dried for 24 h before performing the Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) measurements to decrease the influence of moisture content

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Summary

Introduction

The decay process of fruit is caused by infection and reproduction of microorganisms [1]. Hydrogel antibacterial agent is a synthetic or natural biological material, which is an ideal antibacterial material [4,5]. Of CS–Ag complex hydrogels for controlling postharvest diseases in fruit. There are few reports on the direct application application of CS–Ag complex hydrogels for controlling postharvest diseases in fruit. In this communication, the CS–Ag hydrogels were prepared using the physical crossIn this communication, the CS–Ag hydrogels were and prepared using the physical linking method and characterized by UV-Vis, FTIR, SEM, XRD. The inhibitory effect of the CS–Ag complex gel on fungi the underlying mechanism of the CS–Ag gel against the fungi were explored. The underlying mechanism of the CS–Ag gel against the fungi were explored

(Supplementary
The time-dependent
FTIR spectra powders andpowders
Inhibition of CS–Ag
Effect of CS–Ag on Fungal
Fruit Material and Chemical Material
Fungal Isolation, Identification, Culture, and Spore Suspension
Characterization
Preparation of CS–Ag Complex Hydrogel and CS–AgNPs Sol
Antibacterial Experimental Method
Control of CS–Ag Hydrogel on Fungal Disease In Vivo
Preservation Test of CS–Ag Hydrogel
Morphology of Fungal
Residue of Silver in Grape
Conclusions
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