Abstract

 
 
 
 Purpose: To investigate the therapeutic influence of tanshinone IIA on human bladder cancer cell J82, and the possible signal route involved.
 Methods: Cell proliferative potential was measured using MTT assay, while the expressions of associated genes were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunoblot assays.
 Results: Tanshinone IIA decreased J82 cell survival rate by > 42 % and inactivated apoptosis by suppressing PI3K/AKT/mTOR signal route. Moreover, it decreased Bcl-2, but upregulated caspase and Bax (p < 0.05).
 Conclusion: The inhibitory effect of TIIA on human bladder cancer suggests that TIIA can be developed into an anti-tumor agent.
 
 
 
Highlights
Salvia miltiorrhiza is a rich source of the diterpenoid quinone tanshinone 11A (T11A) [1]
30 μg protein samples were resolved on SDS-PAGE, followed by transfer to PVDF which was subsequently blocked by incubation for 1 h with 5 % de-fatted milk solution in TBST. This was followed by incubation of the membrane overnight with 1o antibodies for pAKT, p-PI3K, and p-mTOR at 4 °C
Treatment with T11A markedly suppressed J82 cell growth in a dose-based fashion, with 80 μg/ml Tanshinone IIA producing about 56.79 % growth inhibition (Figure 1)
Summary
Salvia miltiorrhiza is a rich source of the diterpenoid quinone tanshinone 11A (T11A) [1]. Recent investigations have further revealed that tanshinones have antitumor [5], antioxidant [6], and anti-inflammatory properties [7]. Urothelial bladder carcinoma (UBC) is the most frequently occurring cancer of the urinogenital system [8]. Bladder carcinoma is the 6th most common malignancy in men, and the most frequent urologic cancer [10,11]. There has been a steady upsurge in cases of bladder carcinoma in China [11]. This study was focused on the anticarcinogenic influence of TIIA on human bladder cancer cell J82, and to unravel the possible underlying mechanism. Human bladder cancer cell J82 was kindly. The test for cytotoxic effect of T11A on J82 cells was done using MTT method. Values of p < 0.05 were assumed to be statistically significant
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