Inhibitory effect against reverse transcriptase of HIV-1 of the extract of selected medicinal plants

Abstract

HIV is a virus that multiplies inside of your body and impairs your defences. This makes it more difficult to fend against common illnesses and bacteria. The study's goals included preparing a crude extract from specific medicinal herbs and determining its ability to inhibit HIV-I reverse transcriptase in vitro. This study aimed to conduct an ethnobotanical survey of the healing plants used by Telangana state's indigenous people. Five distinct medicinal plant parts were chosen from a variety of plants, and the % yield was measured after sequential maceration with the five different solvents n-hexane, chloroform, ethyl acetate, acetone, and methanol. Using the RetroSys kit, the HIV reverse transcriptase enzyme inhibition was carried out on crude extracts. Withania somnifera demonstrated excellent inhibitory activity with a range of 40.5 percent to 72.5 percent from a concentration of 0.62 mg/ml to 10 mg/ml, which is close to nevirapine, the control, at 75.3 percent and an effective IC50 value of 1.40 mg/ml used as a lead molecule. HIV-RT inhibition for the aforementioned plants was determined with a range of 32.5 to 72.8 percent.