Abstract
Publisher Summary This chapter discusses the inhibitory and stimulatory effects of Vanadate on sodium pump of Cu ured heart cells from different species. Vanadate (Na3V04) is a potent inhibitor of isolated Na, K–ATPase. In agreement with this finding, active K+ uptake is inhibited in human erythrocytes. In rat adipocytes, however, uptake is unaltered by Na3V04, whereas in cultured rat heart cells, even stimulation has been observed. Experiments have been carried out at 37°C with primary cultures of beating heart muscle cells from neonatal rats, with Girardi cells, an epitheloid-like growing transformed cell line from human heart, and with nonmuscle cells obtained from hearts of neonatal rats and guinea pigs and chicken embryos. Na3V04 inhibits enzyme activity of Na, K–ATPase enriched cell membrane fractions originated from hearts of neonatal rats. In contrast to this finding, a concentration-dependent stimulation of 86Rb+ + K+ uptake has been observed in cultured rat heart muscle cells derived from the same tissue.
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