Inhibitory activity of Socheongryong-tang and its constituent components against the production of RANTES, eotaxin, eotaxin-3 and MMP-9 from BEAS-2B cells

Abstract

Socheongryeong‑tang (SCRT) is a herbal formula previously used to treat pulmonary diseases primarily caused by the common cold virus, including airway inflammation, asthma and allergy. The aim of the present study was to investigate the inhibitory effect of SCRT water extract and its 13 constituent components on chemokine and enzyme production in the human bronchial epithelium cell line BEAS‑2B when induced by tumor necrosis factor‑α and interleukin‑4. The chemokines examined included regulated on activation of normal T‑cell‑expressed‑and‑secreted (RANTES), eotaxin and eotaxin‑3. The SCRT water extract demonstrated a dose‑dependent inhibition of RANTES, eotaxin, eotaxin‑3 and matrix metalloproteinase‑9 (MMP‑9) in BEAS‑2B cells. The 13 constituent compounds of SCRT water extract were quantitatively determined, and it was found that gallic acid, 6‑gingerol and methyl eugenol produced the most potent inhibition of RANTES, eotaxin and eotaxin‑3 as well as MMP‑9 activity regardless of their concentration in SCRT water extract. Principal component analysis and hierarchical clustering analysis revealed that the inhibitory effect of these three compounds contributed to that of SCRT water extract. In conclusion, the results of the present study indicated that the inhibitory effects of SCRT on chemokine and enzyme production in BEAS‑2B cells was associated with three of its constituent compounds, gallic acid, 6‑gingerol and methyl eugenol. This therefore suggested the potential use of these compounds as anti‑inflammatory agents.