INHIBITORY ACTIVITY, METABOLITE CONTENTS DETERMINATION, AND IN SILICO PREDICTION OF PARSLEY LEAVES FRACTION (Petrocelinum crispum MILL) AS ANTIFUNGAL AGENT OF Malassezia furfur

Abstract

Petrocelinum crispum leaves have Furocoumarin as an antimicrobial agent. In this present research, we examine the inhibitory activity of Parsley leaves fractions against Malassezia furfur by disc diffusion paper methods, determine the metabolite contents by LC-MS, and predict the biological activity and toxicity by in silico PASS Prediction. The inhibitory activity of both ethyl acetate and hexane fractions gave medium inhibition at concentrations of 0.15g/ml and 0.3g/ml, respectively. The metabolite contents were revealed by LC-MS, and the PASS prediction showed that the metabolite compounds identified have good biological activity and the xanthotoxin compound has shown nontoxic effects on the skin surface. Concluded that the ethyl acetate fraction showed better inhibition on the growth of Malassezia furfur by moderate category. The stigmastan-3,6-dione and aviprin metabolites were considered the most important metabolites on inhibitory activity predict by PASS prediction, and the xanthotoxin was considered important because of the non-toxic effects