Abstract
During thrombin-induced serotonin release from platelets, phosphorylation of myosin light chain was increased. This protein phosphorylation, which is initiated by Ca 2+ mobilization, and serotonin release were inhibited progressively by increasing concentrations of sodium nitroprusside, a potent stimulator of platelet guanylate cyclase, or 8-bromo-cyclic GMP. Thrombin-induced increase in cytoplasmic free Ca 2+, which was measured with the fluorescent Ca 2+ indicator quin2, was also inhibited by these drugs in a dose-dependent manner. These results provide evidence that an increase in platelet cyclic GMP inhibits thrombin-induced Ca 2+ mobilization and prevents platelet activation.
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