Inhibitor of renal gluconeogenesis (IGN): Additional physiological modulator?

Abstract

1. 1. A procedure for the partial purification of IGN has been developed: it is insoluble in 80% ethanol but soluble in 6% TCA. It moves in void volume on a column of Sephadex G 25, passes through a membrane with cut-off at 25,000 dallons but not through a membrane with cut-off at 10,000 daltons. 2. 2. IGN inhibits gluconeogenesis both in kidney cortex and liver slices. The inhibition is small during the first 30 min of incubation but it increases during the following periods of time. Liver slices are much less sensitive to the inhibitory action of IGN than kidney cortex slices. 3. 3. IGN exerts its effect even in the absence of either K +, Ca 2+ or Mg 2+ from Krebs-Ringer bicarbonate.