インフルエンザウイルスの種々なるinhibitorに関する研究 第5報

Abstract

In detecting antibodies against influenza virus, hemagglutination inhibition titration still remains as a most handy and useful procedure. However, as many one noticed at the outbreak of Asian flu, precautions must be taken in order to destroy non-specific inhibitors contained in the serum specimens, particularly when Asian P virus was used as antigen. The urgent problem at the time of outbreak was dissolved firstly by using KIO4 to remove these inhibitors. However, some destruction of antibody was recognized with this technique. The work here presented was carried out to establish_ the standard procedure to destroy all kinds of non-specific inhibitors but not antibodies.The result pointed out that when a large amount of cholera filtrate (No 558) was added to the specimen and left over night at 37°C, all inhibitors were removed as was the case with KIO4 treatment. Moreover, it was concluded that the cholera filtrate is more suitable for general purposes, i.e. to destroy inhibitors of the serum or organs of experimental animals except serum of guinea pig.As already noticed, inhibitors against Asian P virus detectable in human serum (tentatively called as α′-inhibitor) was resistant to RDE action, but susceptible to the action of cholera filtrate. Thus the difference between cholera filtrate and RDE was studied further, and the term α′-enzyme was proposed to the particular agent which destroys α′-inhibitor or inhibitors. The kinetic study revealed difference between α- and α′-enzyme. The necessity of Ca ion as the co-factor was also different. However, heat lablity of α′-enzyme was most remarkable in differentiating it from α-enzyme. Electrophoretic mobility was also somewhat different.Production conditions and standardization procedure of the cholera filtrate were also described. When the experimental conditions here described was followed carefully, the production of RDE (α-enzyme), α′-enzyme and β-enzyme of the filtrate was constant during the experimental period of 6 months.