Inhibitions inflammatory response in clicks alleviates LPS induced myocardial injury by total polysaccharides of Pinus massoniana Lamb. pollen

Abstract

Total polysaccharides of Pinus massoniana Lamb. pollen (PPPSt) is a natural carbohydrate with known anti-inflammatory effects. Inflammation was the main cause of death due to myocardial damage caused by sepsis. The aim of this study was to investigate the potential protective effect of PPPSt on the septic myocardial injury. Lipopolysaccharide (LPS) was injected intraperitoneally to establish the myocardial injury model and PPPS was used for intervention, then the changes in myocardial enzymes, myocardial tissue morphology, oxidative stress, and inflammation levels were evaluated experimentally. The results showed that PPPSt could alleviate the LPS-induced increase of aspartate transaminase (AST), alanine transaminase (ALT), and creatine kinase (CK) levels, and also ameliorate the histopathological damage of myocardium, but PPPSt had no protective effect on oxidative stress injury. In terms of inflammation, PPPSt treatment can significantly reduce the levels of IL-1β and NO in the serum. In addition, PPPSt can also down-regulate the phosphorylation of Nuclear factor kappa beta (NFκB) and NFκB inhibitor alpha (IKBα) in the septic myocardial tissue, and inhibit the activation of NFκB signaling pathway. In conclusion, PPPSt can protect against LPS-induced septic myocardial injury in chicks, which provides a theoretical basis for the potential treatment of septic myocardial injury.