Inhibition studies of HBV DNA polymerase using seed extracts of Pongamia pinnata.

Manikannan Mathayan,Arumugam Suresh,Selvaraj Jayaraman,Langeswaran Kulanthaivel

doi:10.6026/97320630015506

Manikannan Mathayan, Arumugam Suresh + Show 2 more

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https://doi.org/10.6026/97320630015506

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Abstract

Several antiviral compounds for HBV have been identified from traditionally used medicinal plants. We have earlier described the immune modulation properties of P. pinnata, a traditionally used Indian medicinal plant. Therefore, it is of interest to explore the anti-Hepatitis B virus activity of P. pinnata extracts by in-vitro screening assays. This study clearly demonstrated that the 5mg/ml concentration of the aqueous extract significantly inhibited the virus binding. Further, the spectral study was carried out for finding active compounds. The active chalcone derivatives namely, glabaarachalcone, and isopongachromene were isolated from P. pinnata aqueous seed extracts by standard spectral procedures. Virtual screening data shows that glabaarachalcone, and isopongachromene bound with HBV DNA polymerase protein target.

Highlights

Though viruses are smaller in size and appear simple by their structure, the diseases caused by viruses are complex and devastating and if untreated could be fatal
The anti Hepatitis B virus (HBV) activity of 5 mg extract of P. pinnata seed was treated with varying concentrations of the virus (3 pg/ml up to 0.04 pg/ml) (Figure 1)
It was seen that P. pinnata seed extracts were found to exhibit significant anti HBs Ag activity

Summary

Introduction

Though viruses are smaller in size and appear simple by their structure, the diseases caused by viruses are complex and devastating and if untreated could be fatal. Virus infections range from milder flu like diseases to deadly hemorrhagic fevers, which are extremely fatal [1]. One of the structurally simple and dreadful disease causing virus is Hepatitis B virus (HBV), which is a small circular DNA virus containing a nucleocapsid and an envelope. HBV causes jaundice and in majority of the individuals the primary infection is self-limited and no complication is noticed. If the infection becomes chronic it developed as fatal disease. In India more than 37 million HBV carriers and contributes a large proportion of this HBV burden [2]

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