Inhibition, Recovery and Stabilization of MBR-Anammox Process: Enhancing Nitrogen Removal by Biochar-supported Nano Zero-valent Iron Adoption

Abstract

Anaerobic ammonium oxidation (Anammox) is recognized as a promising option to energy-neutral wastewater treatment, yet difficulties encountered in practice due to its poor stability. This work investigated the effects of biochar-supported nano zero-valent iron (nZVI-BC) on nitrogen removal performance of anammox process. Three membrane bioreactors (MBRs) suitable for slowly growing anammox bacteria was operated for 100 days, undergoing startup, dissolved oxygen (DO) inhibition, performance recovery and stabilization periods. The results showed that nZVI-BC adoption could improve the nitrogen removal efficiency (NRE) by 5.5–27.3 % and 3.1–20.7 % and nitrogen removal rate (NRR) by 5.4–28.6 % and 3.2–22.9 %, compared with control group and biochar adoption, respectively. Throughout the experimental periods, nZVI-BC was conducive to enrich anammox bacteria and increased the functional gene copies associated with anammox metabolism. Meanwhile, co-occurrence network analysis promulgated that nZVI-BC boosted the robustness and interaction of microbial community. Furthermore, the electrochemical analysis of the extracellular polymeric substance (EPS) revealed that nZVI-BC adoption augmented the extracellular electron transport (EET) process of anammox biomass. Metagenomic sequencing revealed that nZVI-BC reinforced the complete dissimilatory nitrate reduction to ammonium (DNRA) pathway in Candidatus Brocadia, the dominant anammox bacteria, potentially reducing nitrate production and enhancing nitrogen removal. The study manifested the possibility of nZVI-BC to improve the anammox robustness and nitrogen removal performance, and provided operational guidance for its engineering application.