Inhibition of V.parahaemolyticus infection using the commensal E.coli HS in a tissue culture infection model

Abstract

V. parahaemolyticusis a Gram-negative pathogenic bacterium. Its pathogenicity contributes to extracellular products(hemolysin) which considered as the most important virulence factor and controls of many biological activities like cytotoxicity, hemolytic activity, enterotoxicity. This bacteriumis able to attach to different types of host cells and cause infection.This study was done to investigate the ability of the well-known commensal bacterium E. coli HS to inhibit the cytotoxic effects and the attachment of V. parahaemolyticus using Hela cell line. The commensal E. coli HS can strongly adhere to epithelial cells without any harm effects. The results revealed that the pre- incubation of Hela cells with the commensal E. coli HS leads to inhibit the cytotoxicity and the attachment of V.parahaemolyticusto Hela cells. Since, bacterial attachment to host cells is a critical stage towards pathogens infection, herein; we conclude that the inhibition of pathogens attachment to host cells may result in inhibition of infection.