Inhibition of Transport Processes of Intestinal Segments Following Augmentation Enterocystoplasty in Rats

Abstract

Purpose Sodium and chloride transport was measured by using the Using chamber-voltage clamp technique to evaluate alterations occurring in ileal segments interposed in the urinary tract in a rat enterocystoplasty model. Materials and Methods In adult male Sprague-Dawley rats which had undergone augmentation ileocystoplasties 3 months earlier, intestinal segments were explanted from the neobladders and native ileal segments were removed separately for electrolyte transport and morphometric analysis. Results Basal ileal active sodium and chloride absorption and cyclic nucleotide-induced chloride secretion were attenuated in transplanted ileum in contrast to control tissue. Morphologically, there was villus epithelial hyperplasia, crypt hyperplasia and chronic inflammation in transplanted segments, but villus height:crypt length ratios were unchanged. Conclusions Pathophysiologic derangements in intestinal segments interposed in the urinary tract include inhibition of sodium and chloride transport processes and mucosal hyperplasia. The use of the Using chamber/voltage clamp technique described herein offers a new approach for additional studies of the metabolic consequences following urinary diversion.