Abstract
EXTRACTS of some varieties of the bean Phaseolus vulgaris can agglutinate erythrocytes1, precipitate human cell homogenates and serum proteins2, stimulate mitosis in leucocytes3, alter the sensitivity of leucocytes to cytotoxic agents and radiation4,5, and stimulate leucocytes in vitro to produce gamma globulin6. A commercially prepared extract of Phaseolus vulgaris, derived according to the method of Rigas and Osgood7, is available as phytohaemagglutinin (PHA-M, Difco Laboratories, Detroit, Michigan). This preparation, which is apparently a mixture of proteins8, is frequently used as a mitogenic agent for in vitro leucocyte cultures. Some of its antigenic properties have already been reported9.
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