Abstract
Two pathways have been implicated in the regulation of maize ferritin synthesis in response to iron. One of them involves the plant hormone abscisic acid (ABA) and controls the expression of ZmFer2 gene(s). Another pathway, ABA-independent, has been characterized in a de-rooted maize plantlet system and involves an oxidative step. The ZmFer1 maize ferritin gene is not regulated by ABA, and it is shown in this paper that the corresponding mRNA accumulates in de-rooted maize plantlets and BMS (Black Mexican Sweet) maize cell suspension cultures in response to iron via the oxidative pathway described previously. To investigate ZmFer1 gene regulation further, the BMS cell system has been used to develop a transient expression assay using a ZmFer1-beta-glucuronidase fusion. Both iron induction and antioxidant inhibition of ZmFer1 gene expression were observed in this system. Using Northern blot analysis and transient expression experiments, it was shown that both okadaic acid and calyculin A, two serine/ threonine phosphatase inhibitors, specifically inhibit ZmFer1 gene expression. These data indicate that an okadaic acid-sensitive protein phosphatase activity is involved in the regulation of the ZmFer1 ferritin gene in maize cells, and this activity is required for iron-induced expression of this gene.
Highlights
Ferritin synthesis is induced by iron excess, mainly at the translational level in mammalian cells [5], whereas a tran
Iron-induced accumulation of ascorbate peroxidase mRNA was independent of an abscisic acid (ABA) pathway and not affected by NAC treatment, whereas ferritin mRNA accumulation induced by an iron excess was blocked by NAC treatment in this system
The ZmFer1 transcripts are the ferritin transcripts that accumulate in response to iron overload of de-rooted plantlets through the activation of the oxidative and ABA-independent pathway
Summary
Ferritin synthesis is induced by iron excess, mainly at the translational level in mammalian cells [5], whereas a tran-. In this paper, using Northern blot experiments and a transient expression assay, we demonstrate that ZmFer1 is the maize ferritin gene induced in de-rooted plantlets and cell suspension cultures, through the previously identified ABAindependent oxidative pathway. To determine if in de-rooted plantlets the increased abundance of ZmFer1 mRNA is stimulated in response to iron through the previously identified oxidative and ABA-independent pathway, Northern blot experiments were performed using the FM1-specific probe in this system (Fig. 1A).
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