Inhibition of the filamentation of Candida albicans by Borojoa patinoi silver nanoparticles

Marcela Gómez-Garzón,Luz D Gutiérrez-Castañeda,Camilo Gil,Ana P Rozo,Carlos H Escobar,Edgar V Sierra,María E González

doi:10.1007/s42452-020-04103-0

Marcela Gómez-Garzón, Luz D Gutiérrez-Castañeda + Show 5 more

Open Access

https://doi.org/10.1007/s42452-020-04103-0

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Abstract

Candida albicans is fungus capable of changing from yeast to filamentous form when it’s transformed from a normal commensal to an opportunistic pathogen. The development of alternatives that interfere with this transition could be an effective way to reduce candidiasis. In this regard, evaluate the inhibitory effect of two Borojoa patinoi silver nanoparticles (AgNPs) produced by green synthesis at 5 °C and 25 °C on the process of filamentation of Candida albicans. The percentage of inhibition of filamentous forms of C. albicans ATCC10231 and C. albicans SC5314 with AgNPs was determined. Results showed that temperature of synthesis affected both the shape and size of silver nanoparticles synthesized using Borojoa patinoi extracts. The inhibition percentage of filamentous forms of Candida albicans ATCC10231 when treated with silver nanoparticles synthesized at 5 °C was 85.9% and at 25 °C it was 40%. C. albicans SC5314 when treated with AgNP synthesized at 5 °C was 97.2% and at 25 °C it was 64%. Cell toxicity assay showed that at 100ng/ml, AgNPs synthesized at 25 °C were safe in MES-OV CRL-3272 cell line. Our results showed that the silver nanoparticles obtained from Borojoa patinoi are inhibitors of the filamentous process of C. albicans.

Highlights

Candida albicans is a polymorphic fungus, capable of developing reversible morphological changes, between yeast and filamentous form
The purpose of this work was to evaluate the inhibitory effect of silver nanoparticles of Borojoa patinoi produced at 5 °C and 25°C on the process of filamentation of Candida albicans
The formation of nanoparticles due to the reduction of AgNO3 during incubation with Borojoa patinoi extract is evident by the change in the color of the reaction mixture, which turned from yellow to red-brown

Summary

Introduction

Candida albicans is a polymorphic fungus, capable of developing reversible morphological changes, between yeast and filamentous form. This form is acquired when it’s transformed from a normal commensal to an opportunistic pathogen. The bottom up procedure for nanoparticles synthesis, consist of forming metal agglomerates by chemical reducing metal inorganic ions (gold, silver, iron and metal oxides). Have it been proposed that these nanoparticles could be produced harnessing the reducing capacities of metabolites and proteins in biological systems, known as green synthesis. The green synthesis of silver nanoparticles using different biological systems (including viruses, bacteria, fungus and plants), has the advantages of being a simple, safe and low cost Nasrollahzadeh, Mahmoudi-Gom Yek et al [22].

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