Inhibition of suppressor cell generation by mouse serum in culture

Abstract

Normal C57BL/6 mouse spleen cells cultured for five days in the presence of fetal calf serum (FCS-induced suppressor generation culture) were shown, in mixing experiments, to suppress the primary humoral response of freshly explanted C57BL/6 spleen cells against sheep erythrocytes (SRBC). This suppressor cell generation was largely dependent on the FCS concentration in the suppressor generation culture. Ten or 5% FCS effectively supported the generation of suppressor cells, but 1% FCS only marginally supported it. When mouse serum (MS) from normal C57BL/6 mice was added to the suppressor generation culture, it inhibited the generation of the suppressor cells. Sera from allogenic mice and athymic nude mice were also effective. The effect of MS was resistant to heat treatment (56°C, 30 min). The inhibitory activity of MS was not dialyzable, and concentrated into the fraction which was not precipitated by 50% saturation of ammonium sulfate and which was eluted at a concentration of about 0.2 M NaCl from a DEAE-Sephadex A-50 column. The active fraction of MS also effectively inhibited the growth of Ehrlich tumor cells in culture. Further, MS also inhibited the generation of Con A-induced suppressor cells. The inhibition by MS of FCS-induced suppressor generation was eliminated by an interleukin 2-containing preparation.