Inhibition of stimulated osmotic water flow by fluphenazine, a calmodulin inhibitor, in the isolated toad skin

Abstract

1. The effects of fluphenazine, a phenothiazine calmodulin inhibitor, on the osmotic water flow ( P osm) across isolated skins of Bufo arenarum toads were tested. 2. Fluphenazine inhibited the increase in P osm induced by agents known to act through the cyclic AMP system (oxytocin, db-cAMP, theophylline, KCl and isoproterenol). 3. The inhibitory effect was faster and more intense when the drug was present in the epidermal bath, and persisted after rinsing the preparation for 100 min. 4. Our results indicate that phenothiazine's action may be primarily exerted at a site distal to cyclic AMP generation.