Abstract
In the assay of serum PON arylesterase activity using phenyl acetate as a substrate, a number of other serum hydrolases can catalyze the substrate hydrolysis, and so bring high background activity signal. EDTA and 2-hydroxyquinoline (2-HQ) were PON inhibitors. Based on this property, background activity can be determined by adding EDTA or 2-HQ just prior to adding the substrate (phenyl acetate). The specific serum PON activity was obtained by subtracting the background activity from the total serum arylesterase activity. The correlation of the background signals based on different inhibitors and the correlation of the specific serum PON activity were investigated, respectively. The inhibition consistency was analyzed. The correlation analysis of PON activity-age was performed.
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