Inhibition of receptor-mediated endocytosis immunoglobulin G by enterocytes isolated from the neonatal rat jejunum

Abstract

Abstract The effect of inhibitors of respiration (NaN 3 and DNP), glycolysis (2DG, IAA and NaF) and the microtubular-microfilament system (colchicine and cytochalasin B) on the uptake of rat immunoglobulin G (IgG) by enterocytes isolated from the neonatal rat gut has been assessed. After a 1 hour incubation, NaN 3 and DNP had significantly reduced IgG uptake by between 32% and 35% of the control, IAA and 2DG were less effective and NaF, colchicine and cytochalasin B had no effect at all. The findings show that (i) IgG is internalised by isolated enterocytes in vitro and (ii) that this internalisation is under metabolic control, (iii) that inhibitors of respiration are more effective in blocking uptake than inhibitors of glycolysis.