Abstract
This study was conducted to analyse extracts and essential oils of Ferulago macedonica and Echinophora sibthorpiana (Apiaceae) for anti–quorum sensing potential using Pseudomonas aerugonisa PAO1 as biomonitoring system. Anti–quorum sensing screening was carried out by tree different tests which showed that examined samples possessed significant effects in suppressing of some virulent determinants of PAO1. The essential oils of F . macedonica and E . sibthorpiana aerial parts reduced the growth and modified the appearance of P . aeruginosa colonies and flagella to a large extent in twitching and motility assay. In the presence of essential oil of E . sibthorpiana , diameter of the colony was 6.67 mm, the flagella were absent and the color of the colony was changed from green to white. In anti–biofilm assay, the inhibitory activity of E . sibthorpiana was more notable than of F . macedonica . Applied colorimetric test revealed that examined samples strongly reduced the production of pigment pyocyanin where the highest inhibition of its synthesis showed F . macedonica oil (5.22%), followed by E . sibthorpiana oil (10.69%), which was singnificantly bigger potential in comparison to used antibiotics (141.55%).
Highlights
Studied species Ferulago macedonica Micevski et Mayer and Echinophora sibthorpiana (Guss.) Tutin are herbaceous perennial plants which belong to the carrot family (Apiaceae)
The aim of this study was to determine the anti-quorum sensing activity of F. macedonica and E. sibthorpiana on P. aeruginosa PAO1 taking into consideration the widespread use of related representatives as antimicrobial agents in traditional medicine
Only a few species were explored for QS potential (Khan et al, 2009; Sepahi et al, 2015) and this is the first data on F. macedonica and E. sibthorpiana activity against QS communication in P. aeruginosa
Summary
Studied species Ferulago macedonica Micevski et Mayer and Echinophora sibthorpiana (Guss.) Tutin are herbaceous perennial plants which belong to the carrot family (Apiaceae). The aim of this study was to determine the anti-quorum sensing (anti–QS) activity of F. macedonica and E. sibthorpiana on P. aeruginosa PAO1 taking into consideration the widespread use of related representatives as antimicrobial agents in traditional medicine. This analysis was conducted by determining the ability of extracts and essential oils to reduce twitching and flagella motility, biofilm formation and pyocyanin production of this bacterium and the comparison of their activity with the effects of commercial antibiotics was done
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