Abstract

An immunocytochemical stain was developed to detect the basidiomycete A. bombacina in apple leaf litter. The polyclonal antibodies for A. bombacina were sufficiently specific that only hyphae of this fungus were detected in immunocytochemically treated sections of dead leaves inoculated with A. bombacina. Apple leaves naturally infected with V. inaequalis were inoculated with A. bombacina, incubated outside from November to May, and sampled monthly

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