Abstract
Photosystem II (PS II) activity (pBQ-supported) in Anacystis nidulans was inhibited by copper concentrations of 5, 10 and 15 μM; the F v F m ratio also decreased. The decrease in F m was due to a decrease in F v, thus showing that at all the concentrations tested copper affects PS II photochemistry. Higher concentrations of copper (10 and 15 μM) completely quenched the variable fluorescence of the cells. Fluorescence spectra of intact cells showed a shift in chlorophyll peak indicating that 15 μM copper inhibits PS II activity by causing structural alterations in the Chl-proteins of the PS II complex. The absorption and fluorescence spectra also indicated that 5, 10 and 15 μM of copper cause bleaching of phycocyanin, thereby affecting the light harvesting complex as well.
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