Inhibition of propolis and trigona spp's honey towards Methicilin-Resistant staphylococcus aureus and Vancomycin-Resistant staphylococcus aureus

Abstract

Propolis and Trigona spp honey have functioned as anticancer, antiviral, antifungal and antibiotic. Isolates of Staphylococcus aureus resistant to Methicillin and Vancomycin found in the surgical treatment room and ICU of Ratu Zalecha Hospital Martapura. The purpose of this research was to determine the inhibitory zone of MRSA, VRSA to the propolis extract and honey of Trigona spp at the concentration of 200 mg/ml, 400 mg/ml, 600 mg/ml, 800 mg/ml and 1000 mg/ml. Also to know the concentration of the propolis extract and honey of Trigona spp in inhibiting the growth of MRSA, VRSA. The type of this research was true experimental with the design of Pretest-Posttest With Control Group Design. The study was conducted from April 2015 to June 2015 in Banjarbaru, South Kalimantan Indonesia. The objects of research are propolis and honey of Trigona spp hives in the Barabai area. The dependent variable was the inhibition zone of MRSA and VRSA in Hinton Muller` media containing ethanol extract of propolis and honey of Trigona spp in some different concentrations measured from the formed diameter of inhibition zone. Data were analyzed by One Way ANOVA test and Kruskal-Wallis test at 95% confidence level. The results of the research showed that there were differences in MRSA and VRSA inhibition zone against various concentrations of Trigona spp honey. The strength of Trigona spp honey which was in inhibiting the growth of MRSA was 1000 mg/ml concentration with a diameter of 25.2 mm. VRSA at 1000 mg/ml concentration with a diameter of 26.6 mm. The strength of Trigona spp propolis extract which is useful in inhibiting the growth of MRSA is at 1000 mg/ml concentration with a diameter of 17.8 mm. VRSA at 1000 mg/ml concentration with a diameter of 16.4 mm.