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Abstract
Ovarian carcinoma has a cure rate of 30% which makes it deadlier than any other disease. There are a number of genetic and epigenetic changes that lead to ovarian carcinoma cell transformation. Chemoprevention of cancer through application of natural compounds is the need of present generation as other methods are rigorous and have many side effects. Betanin, a compound from Beta vulgaris extract is used in present study to check its potential for inhibition of (PA-1) cancer cell proliferation. Determination of IC50 values through MTT assay was carried out, in addition measurement of mitochondrial membrane potential (MMP), effect of reactive oxygen species (ROS) generation, and induction of apoptosis in ovarian cancer cells through betanin was also observed. Results have shown betanin as a potential candidate for inhibition of ovarian cancer cell proliferation and it can be taken up as a serious compound for further studies for its application in cancer cure.
Highlights
Most death among women is caused by ovarian cancer
Materials And Methods: Source of reagents and chemicals: Betanin (Red Beet extract diluted with Dextrin) purchased from TCI Chemicals, streptomycin, penicillin-G, Dulbecco’s Modified Eagle’s Medium (DMEM), L-glutamine, 3-(4,5 dimethylthiozol-2-yl)-2,5diphenyltetrazoliumbromide, acridine orange (AO), phosphate buffered saline, bovine serum albumin, ethidium bromide (EB), 2’7’diacetyl dichloro fluorescein, ethanol, trypan blue, triton X-100, Rhodamine 123 (Rh-123), dimethyl sulfoxide (DMSO), Dichloro-Dihydro-Fluorescein Diacetate (DCFH-DA) and trypsin-EDTA were bought from Sigma Aldrich chemicals Pvt
The PA-1 cancer cells lines were treated with various concentration of Betanin (10-60μg/ml) for 24hr and the results are expressed as a % of the control value in presenting as a cell cytotoxicity ratio for PA-1 cells using MTT assay (Fig. 2)
Summary
Most death among women is caused by ovarian cancer. Diagnosis of Ovarian Cancer (OC) is very difficult since symptoms are seen only at the later stages. Women from age 20 to 55 are largely affected. More than 60% of patients with OC have shown symptoms of recurrence even after surgery. Major treatments for cancer include radiotherapy, chemotherapy and surgery. The cancer cells are affected by chemical agents and through apoptotic program induction the cell is destroyed. Growing cancer cells as well as normal cells are targeted by classic chemotherapeutic agents that cause side effects in the patients [1]. Worldwide with the increase in cancer cases, development of novel, potent and safe anticancer agent is still a serious challenge
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