Inhibition of pro-inflammatory mediators in RAW264.7 cells by 7-hydroxyflavone and 7,8-dihydroxyflavone.

Abstract

Flavonoids are a class of compounds that having the benzo-γ-pyrone skeleton, which possess anti-inflammatory properties in vitro and in vivo. The aim of this study was to investigate the inhibition of two flavonoids 7-hydroxyflavone (HF) and 7,8-dihydroxyflavone (DHF) on the production of pro-inflammatory mediators in RAW264.7 cells activated by lipopolysaccharides (LPS). For this purpose, we selected four pro-inflammatory mediators including nitric oxide (NO), prostaglandin E2 (PGE2 ), tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) as markers to evaluate the anti-inflammatory activity of HF and DHF. In this regard, we showed that HF and DHF dose-dependently reduced the production of NO, PGE2 , TNF-α and IL-6 through downregulating mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX2), TNF-α and IL-6, respectively. Moreover, DHF generally possesses more efficient than HF in reducing these markers secretion in this study. Consider together, these findings suggest that DHF and HF can inhibit LPS-induced inflammation via attenuating the production of NO, PGE2 , TNF-α and IL-6, indicating that they may be lead compounds for developing anti-inflammatory agent.