Abstract
Seed extracts of Dianthus barbatus L. inhibited the development of local lesions induced by tobacco mosaic virus-ordinary strain (TMV-OM) and potato virus X (PVX) on Chenopodium amaranticolor Coste et Reyn. when mixed with the inocula. The extracts prevented infection of Samsun NN tobacco and French bean by TMV-OM when applied within 3 days before or within 3 hours after application of TMV-OM inocula. The extracts also showed virus inhibition when they were applied to the lower surface of leaves of Samsun NN tobacco and French bean and then TMV-OM was applied to the upper surface. The inhibitory activity could be separated into 3 distinct substances by gel-filtration chromatography on a column of Sephadex G-25. Strong inhibitory activity was observed in the substance of high molecular weight. A purification procedure of the inhibitive substance will be reported in the next paper.
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