Inhibition of photosynthetic activity by UV‐B radiation in radish seedlings

Abstract

Inhibition of primary photosynthetic reactions by UV‐B radiation (280 nm‐320 nm) was demonstrated in radish leaves (Raphanus sativus cv. Saxa Treib). Detached radish cotyledons from 10‐day‐old seedlings were irradiated with continuous white light and increasing UV‐B irradiances using cut‐off filters with increasing transmission for shorter wavelengths (WG 360, WG 345, WG 320, WG 305, WG 295, WG 280). Photosynthetic activity measured in terms of chlorophyll fluorescence induction (Kautsky effect) after 2, 4, 6, 8 and 24 h irradiation decreased in a wavelength dependent way with increasing UV‐B irradiance and irradiation time.Radish seedlings grown for 10 days from the time of germination under the same UV‐B irradiation conditions exhibited similar reductions of the variable fluorescence as detached cotyledons irradiated for short time periods. They additionally had lower initial fluorescence at high UV‐B radiation levels, although the chlorophyll content per leaf area increased. In contrast to short term experiments, the plastoquinone and flavonoid content increased with increasing UV‐B irradiance when based on leaf area.