Abstract
BackgroundLactobacillus spp. predominantly shows its presence as a normal mucosal flora of the mouth and intestine. Therefore, the objective of our research is to investigate the in-vitro conditions for the prospective of medically valuable biosurfactants (BSs) derived from Lactobacillus spp. Biosurfactant (BS) obtained from Lactobacillus spp. exhibit antibiofilm and antiadhesive activity against broad range of microbes. In the present study we investigated the production, purification and properties of key components of the cell-associated-biosurfactant (CABS) from Lactobacillus acidophilus NCIM 2903.ResultsExtracted, purified, freeze-dried CABS shows reduction in surface tension (SFT) of phosphate buffer saline (PBS @pH 7.0) from 71 to 26 mN/m and had a critical micelle concentration (CMC) of 23.6 mg/mL. The CABS showed reduction in interfacial tension (IFT) against various hydrocarbons and had effective spreading capability as reflected through the decrease in contact angle (CA) on different surfaces (polydimethylsiloxane - PDMS, Teflon tape, glass surface, polystyrene film and OHP sheet). The anionic nature of CABS displayed stability at different pH and temperatures and formed stable emulsions. Thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) revealed CABS as glycolipoprotein type. The Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed presence of multiple bands in a molecular range of 14.4 to 60 kDa, with prominent bands of 45 kDa. The CABS has significant antiadhesion and antibiofilm activity against tested bacterial strains.ConclusionThe current challenging situation is to develop methods or search for the molecules that will prevent the formations of biofilm on medical bioimplants of PDMS based materials. These findings are supportive for the use of Lactobacilli derived BS as potential antiadhesive agent on various surfaces of biomedical devices.
Highlights
Lactobacillus spp. predominantly shows its presence as a normal mucosal flora of the mouth and intestine
Medium for biosurfactant synthesis BS produced by L. acidophilus National Collection of Industrial Microorganisms (NCIM) 2903 was determined by measuring the surface tension (SFT) of cell free broth and in case of cell bound BS extracted in Phosphate buffer saline (PBS)
L. acidophilus did not synthesized BS in MRS and MRS-T evident from SFT values. Both MRS and MRS-T supported the growth of L. acidophilus culture; no drastic change in pH was observed (Refer Fig. 1)
Summary
Lactobacillus spp. predominantly shows its presence as a normal mucosal flora of the mouth and intestine. Biosurfactants (BSs) are amphiphilic molecules produced by diverse microorganisms such as bacteria, fungi and yeasts at the microbial cell surface or excreted out with pronounced surface and emulsifying activities [1,2,3]. These molecules possess a tendency to accumulate at the interface between liquid phases that show different degrees of polarity and hydrogen bonding, like oil-water or air-water, and Satpute et al BMC Microbiology (2019) 19:39 insecticidal agents in therapeutics. In this study, we explored the BS from L. acidophilus NCIM 2903 and structurally characterized for antibiofilm properties using various model organisms
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